Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Types of RNA

Types of RNA

Overview
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in the regulation of gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

RNA Interference

RNA Interference

RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...

siRNA - Small Interfering RNAs

siRNA - Small Interfering RNAs

Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the ATP-dependent...

Experimental RNAi

Experimental RNAi

RNA interference (RNAi) is a cellular mechanism that inhibits gene expression by suppressing its transcription or activating the RNA degradation process. The mechanism was discovered by Andrew Fire and Craig Mello in 1998 in plants. Today, it is observed in almost all eukaryotes, including protozoa, flies, nematodes, insects, parasites, and mammals. This precise cellular mechanism of gene silencing has been developed into a technique that provides an efficient way to identify and determine the...

Types of RNA

Types of RNA

Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Effects of Chromatin Structure Modifiers on the trans-Acting Heterochromatin Position Effect in Drosophila melanogaster.

Doklady. Biochemistry and biophysics·2024

Same author

High Level of Gene Transcription at the Embryonic Stage Leads to the Suppression of Heterochromatic Trans-Inactivation in Drosophila melanogaster Adults.

Biochemistry. Biokhimiia·2020

Same author

Nature and Functions of Telomeric Transcripts.

Biochemistry. Biokhimiia·2019

Same author

ROLE OF TELOMERIC PROTEIN COMPLEX AND TELOMERIC RNA IN EARLY DEVELOPMENT OF DROSOPHILA.

Tsitologiia·2018

Same author

[Participation of the piRNA pathway in recruiting a component of RNA polymerase I transcription initiation complex to germline cell nucleoli].

Molekuliarnaia biologiia·2017

Same author

[Nascent Polypeptide-Associated Complex as Tissue-Specific Cofactor during Germinal Cell Differentiation in Drosophila Testes].

Molekuliarnaia biologiia·2017

Search research articles

Related Experiment Video

Updated: Jul 12, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

[RNA binding proteins in the RNA interference phenomenon].

R N Kotel'nikov, S G Shpiz, A I Kalmykova

Molekuliarnaia Biologiia

|August 18, 2006

Summary

Short RNAs, including small interfering RNA (siRNA) and microRNA (miRNA), silence genes through RNA silencing pathways. This review covers their biogenesis and the function of novel RNA binding domains in RNA interference (RNAi).

More Related Videos

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

An Assay for Quantifying Protein-RNA Binding in Bacteria

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

Related Experiment Videos

Last Updated: Jul 12, 2026

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Detection of RNA-binding Proteins by In Vitro RNA Pull-down in Adipocyte Culture

Published on: July 22, 2016

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Novel RNA-Binding Proteins Isolation by the RaPID Methodology

Published on: September 30, 2016

An Assay for Quantifying Protein-RNA Binding in Bacteria

An Assay for Quantifying Protein-RNA Binding in Bacteria

Published on: June 12, 2019

Area of Science:

Molecular Biology
Genetics
Biochemistry

Context:

RNA silencing is a fundamental biological process.
Short RNAs play crucial roles in gene regulation.
RNA interference (RNAi) is a key mechanism within RNA silencing.

Purpose:

To review the biogenesis pathways of small interfering RNA (siRNA) and microRNA (miRNA).
To explore the function of newly identified RNA binding domains in RNA interference (RNAi) proteins.
To describe the interactions between these novel domains and established RNA binding modules.

Summary:

Short RNAs (21-27 nucleotides) mediate gene silencing via homologous sequences.
Biogenesis pathways for siRNA (from double-stranded RNA) and miRNA (from hairpin RNA) are outlined.
The study examines specific RNA binding domains in RNAi proteins and their interactions with known RNA binding modules.

Impact:

Enhances understanding of RNA silencing mechanisms.
Provides insights into the molecular basis of gene regulation by short RNAs.
Identifies novel protein domains involved in RNA interference, potentially opening new avenues for therapeutic development.