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A structural model for the genome of echovirus 22
1Department of Microbiology and Immunology, Louisiana State University Medical Center, Shreveport.
Archives of Virology
|January 1, 1990
Summary
The echovirus 22 genome is a single-stranded RNA with a hairpin structure at the 5'-terminus. It also features a VPg-like protein and a polyadenylic acid tail at the 3'-end.
Area of Science:
- Virology
- Molecular Biology
- RNA Structure
Background:
- Echovirus 22 genome structure was previously hypothesized.
- Further characterization of the viral RNA (vRNA) is needed.
Purpose of the Study:
- To further characterize the structural features of the echovirus 22 genome.
- To confirm the presence of secondary structures and terminal modifications.
Main Methods:
- Digestion with selective ribonucleases.
- Electrophoresis in composite gels.
- Hydrodynamic studies in density gradients (Cs2SO4 and sucrose).
- Thermal denaturation analysis.
- 3 -terminal ribonucleotide analysis.
Main Results:
- Echovirus 22 genome is a single-stranded RNA molecule.
- A stable hairpin secondary structure with hydrogen-bonded base-pairing exists at the 5 -terminus.
- A VPg-like protein is attached to the 5 -end of the RNA.
- A polyadenylic acid tract [poly(A)] is present at the 3 -terminus.
Conclusions:
- The echovirus 22 genome possesses a distinct structural organization.
- The 5 -terminal hairpin, VPg-like protein, and 3 -terminal poly(A) tract are key features of the echovirus 22 RNA structure.