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Updated: Aug 6, 2026
A Duplex Digital PCR Assay for Simultaneous Quantification of the Enterococcus spp. and the Human Fecal-associated HF183 Marker in Waters
Published on: March 9, 2016
Shortcut detection of the vanB gene cluster in enterococci by a duplex real-time PCR assay
1Department of Microbiology, Royal Prince Alfred Hospital, Camperdown, New South Wales, Australia. nick.adams@member.rcpa.edu.au
Aim:
To develop a robust, simple and rapid method for detection of vanB in enterococci.
Methods:
A real-time duplex PCR assay for the simultaneous detection of Enterococcus faecium and vanB resistance genotype in enterococci was developed in conjunction with a simple method for DNA extraction. The assay was tested on 130 fresh plate cultures of clinical isolates of enterococci and other Gram-positive bacteria.
Results:
Forty-eight isolates of vanB E. faecium from 32 different patients and three isolates of vanB E. faecalis were detected within 1 hour. All isolates of E. faecium were identified correctly.
Conclusion:
This simple method for the detection of resistance mediated by vanB is a potentially useful method that is suitable for use in the diagnostic microbiology laboratory.
