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Characterization of adhesion zones in E. coli cells
1Unité associée du CNRS, Biochimie Moléculaire et cellulaire Université Paris-sud, Orsay, France.
FEMS Microbiology Letters
|January 15, 1990
Abstract:
After plasmolysis of Escherichia coli cells, the adhesion zones were characterized using the cytochemical PTA and SP procedures which stain peptidoglycan and lipopolysaccharides (LPS) respectively. A PTA-stained layer was detected at the adhesion sites. This layer was visualized irrespective of the electron microscopy procedure used. Also, using SP staining an outer membrane in which LPS molecules were asymmetrically distributed, was observed.