Related Experiment Video
Updated: Feb 6, 2026
08:25
Author Spotlight: Effective Immunofluorescent Staining Protocol for Intestinal Cell Biology Research
Published on: July 19, 2024
7.1K
A simple rapid method to slice biological specimens: an application for non-embedded and embedded Golgi-stained
E Kolodziejczyk1, P Serrant, M R Fernandez-Graf
1Section of Microscopy, Nestlé Research Centre, Nestec Ltd., Lausanne, Switzerland.
Journal of Neuroscience Methods
|March 1, 1990
Summary
A new rotary wire saw significantly speeds up tissue slicing for histology. This method simplifies preparation for techniques like the Golgi-Cox method, reducing processing time and eliminating freezing steps.
Area of Science:
- Histology and Tissue Preparation
- Neuroscience Techniques
- Biomedical Research Tools
Background:
- Traditional tissue slicing methods can be time-consuming and complex.
- Preserving tissue integrity during slicing is crucial for accurate analysis.
- Established histological techniques often require lengthy preparation steps.
Purpose of the Study:
- To introduce a rotary wire saw as an improved method for tissue slicing.
- To demonstrate the application of the rotary wire saw in conjunction with the Golgi-Cox method.
- To present modifications of the Golgi-Cox method that reduce preparation time.
Main Methods:
- Utilizing a rotary wire saw for slicing both soft and hard biological tissues.
- Applying the rotary wire saw to the Golgi-Cox staining method.
- Modifying the Golgi-Cox protocol to eliminate the need for specimen freezing and embedding.
- Substituting LR-White for celloidin in the Golgi-Cox preparation.
Main Results:
- The rotary wire saw significantly increases the speed of tissue slicing.
- Tissue slicing is markedly simplified using the rotary wire saw.
- No apparent loss of quality in sliced tissues was observed.
- Preparation time for the Golgi-Cox method is reduced.
- Sections ranging from 20 microns to several millimeters thick can be obtained.
Conclusions:
- The rotary wire saw offers a faster and simpler approach to tissue slicing.
- This method maintains tissue quality while streamlining histological preparations.
- The described applications enhance the efficiency of the Golgi-Cox method for research.
More Related Videos
07:41Rigid Embedding of Fixed and Stained, Whole, Millimeter-Scale Specimens for Section-free 3D Histology by Micro-Computed Tomography
Published on: October 17, 2018
9.4K
08:08qPCR Is a Sensitive and Rapid Method for Detection of Cytomegaloviral DNA in Formalin-fixed, Paraffin-embedded Biopsy Tissue
Published on: July 9, 2014
16.3K
Related Concept Videos
01:49Golgi Apparatus
102.5K
As they leave the Endoplasmic Reticulum (ER), properly folded and assembled proteins are selectively packaged into vesicles. These vesicles are transported by microtubule-based motor proteins and fuse together to form vesicular tubular clusters, subsequently arriving at the Golgi apparatus, a eukaryotic endomembrane organelle that often has a distinctive ribbon-like appearance.
102.5K
01:28Biological Methods for Microbial Control
891
Biological agents offer an effective means of controlling microbial growth by leveraging natural processes like predation, competition, and the secretion of antimicrobial substances.Predatory bacteria such as Bdellovibrio species target and kill pathogens like Salmonella and E. coli. They are widely used in poultry farms to control infections. Myxococcus species help combat plant-pathogenic fungi. These naturally occurring predators serve as eco-friendly alternatives to chemical pesticides and...
891
01:24Simple Staining Technique
3.8K
OverviewStaining techniques in microscopy enhance the visualization of microorganisms by increasing contrast and allowing the differentiation of cellular structures. Simple staining is one of the fundamental methods used to observe the basic morphological characteristics of microorganisms, including their size, shape, and arrangement. This method relies on the application of a single dye to stain the entire cell, producing a clear contrast between the cell and the background.FixationFixation is...
3.8K
01:25Applications Of NMR In Biology
4.5K
Nuclear magnetic resonance (NMR) spectroscopy is a very valuable analytical technique for researchers. It has been used for more than 50 years as an analytical tool. F. Bloch and E. Purcell formulated NMR in 1946 and won the 1952 Nobel Prize in Physics for their work. Biological macromolecules such as proteins, nucleic acids, lipids, and organic molecules including pharmaceutical compounds, can be studied using this versatile tool that exploits the magnetic properties of certain nuclei.
4.5K
01:09Golgi Apparatus
21.9K
Properly folded and assembled proteins are selectively packaged into vesicles that exit the ER. Motor proteins transport these vesicles to the Golgi apparatus for adding modifications that make these proteins functional at their destination.
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
The Golgi apparatus is a eukaryotic organelle that has a distinctive ribbon-like appearance. It is a primary sorting and dispatch station for cargo arriving from the ER. Newly arriving vesicles enter the cis face of the Golgi, closest to the ER, and are...
21.9K
01:26Transport Across the Golgi
6.2K
While it is unclear how molecules move between adjacent Golgi cisternae, it is apparent that the molecules move from cis- cisterna, the entry face, to the trans- cisterna, the exit face. Experiments initially suggested vesicles that bud from one cisterna and fuse with the next cisterna to transport proteins between the cisternae. This vesicular transport model describes the Golgi apparatus as a relatively static structure with a unique enzyme composition in each cisterna. Molecules are...
6.2K