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Published on: November 16, 2016

Cytotoxicity and antimicrobial activity of six multipurpose soft contact lens disinfecting solutions

Jacinto Santodomingo-Rubido1, Osamu Mori, Satoshi Kawaminami

  • 1Menicon Co. Ltd, 21-19, Aoi 3 Naka-Ku, Nagoya 460-0006, Japan. j.santodomingo@menicon.com

Ophthalmic & Physiological Optics : the Journal of the British College of Ophthalmic Opticians (Optometrists)
|August 22, 2006
PubMed
Abstract

Insights

Six multipurpose disinfecting contact lens solutions (MPDS) showed varying cytotoxicity and antimicrobial activity. MeniCare Soft demonstrated effective antimicrobial action with minimal cytotoxicity, unlike other tested solutions.

Area of Science:

  • Ophthalmology
  • Microbiology
  • Biocompatibility Testing

Background:

  • Multipurpose disinfecting contact lens solutions (MPDS) are widely used for contact lens care.
  • Assessing the safety and efficacy of MPDS is crucial for preventing ocular complications.
  • Variability in MPDS formulations can lead to differences in performance.

Purpose of the Study:

  • To evaluate and compare the cytotoxicity of six different MPDS.
  • To assess and compare the antimicrobial activity of the same six MPDS.
  • To identify MPDS with optimal safety and efficacy profiles.

Main Methods:

  • Cytotoxicity was determined using the colony-forming assay (ISO 10993-5) with Chinese hamster fibroblast V79 cells.
  • Antimicrobial activity was assessed via the stand-alone contact lens disinfection test (ISO 14729).
  • Six MPDS were tested at various concentrations to determine their effects on cell viability and microbial inactivation.

Main Results:

  • MeniCare Soft and Complete Moisture Plus exhibited no cytotoxicity at tested concentrations.
  • Solo-Care Aqua, Opti-Free Express, ReNu MultiPlus, and ReNuMoistureLoc showed increasing levels of cytotoxicity.
  • All MPDS met bacterial disinfection criteria, but ReNu MoistureLoc had borderline results for certain bacteria, and Complete Moisture Plus failed for Fusarium solani.

Conclusions:

  • Significant variations in cytotoxicity and antimicrobial efficacy exist among MPDS.
  • Solution formulation, not just active ingredient concentration, impacts MPDS performance.
  • MeniCare Soft emerged as a promising option with high antimicrobial activity and low cytotoxicity.

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