Optimization of sample preparation from skin mucus of a neotropical fish for two-dimensional substrate gel

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Cristiane M C de Salles1, Herbert L M Guedes, João B Salles

  • 1Laboratório de Bioquímica de Proteínas e Peptídeos, Departamento de Bioquímica e Biologia Molecular, Instituto Oswaldo Cruz, FIOCRUZ, 21045-900 Rio de Janeiro, Brazil. salles.cristiane@gmail.com

Analytical Biochemistry
|August 22, 2006
PubMed
Abstract

No abstract available in PubMed .

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Two-dimensional Gel Electrophoresis01:22

Two-dimensional Gel Electrophoresis

Two-dimensional gel electrophoresis is a high-resolution protein separation method first introduced by O' Farrell and Klose in 1975. This method involves protein separation by two dimensions, mass and charge, making it more accurate than one-dimensional gel electrophoresis.
The first dimension separation uses the isoelectric focusing or IEF technique performed on immobilized pH gradient (IPG) strips that separate proteins according to their isoelectric points.
Biological samples, such as  cells...