Related Experiment Video
Updated: Aug 6, 2026
In Ovo Xenografting of Patient-Derived Acute Lymphoblastic Leukemia (ALL) Cells (PDX-ALL)
Published on: August 1, 2025
[Asparagine synthetase activity in pediatric acute lymphoblastic leukemia]
Hua Jiang1, Long-Jun Gu, Hui-Liang Xue
1Department of Hematology/Oncology, Shanghai Children's Medical Center Affiliated to Medical College of Shanghai Jiaotong University, Shanghai 200127, China. jiang_hua18@yahoo.com.cn
Objective:
To study the cellular activity of asparagine synthetase in different types of childhood acute lymphoblastic leukemia (ALL).
Methods:
The cellular activity of asparagine synthetase was detected by HPLC-FLD and Protein measurement in 28 ALL children (7 cases of T-ALL and 21 cases of B-lymphoid lineage ALL) before chemotherapy.
Results:
The asparagines synthetase activity levels in T-ALL children were significantly higher than those of the B-lymphoid lineage ALL patients, with the median activity level of 9.3 nM Asn/mg protein/hr vs 5.2 nM Asn/mg protein/hr (P < 0.05). The distribution of the asparagine synthetase activity demonstrated a polymorphism in either T-ALL or B-lymphoid lineage ALL patients.
Conclusions:
The cellular activity of asparagines synthetase in ALL patients is presented with a polymorphism distribution. The asparagines synthetase activity levels in T-ALL are significantly higher than in B-lymphoid lineage ALL.
