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Updated: Aug 5, 2026
A Revised Surgical Approach to Induce Endolymphatic Hydrops in the Guinea Pig
Published on: June 4, 2020
Rupture of Reissner's membrane during acute endolymphatic hydrops in the guinea pig: a model for Ménière's disease?
W L Valk1, H P Wit, F W J Albers
1Department of Otorhinolaryngology, University Medical Center Groningen, Groningen, The Netherlands. w.l.valk@kno.umcg.nl
Conclusion:
The changes in cochlear function during a destructive acute endolymphatic hydrops were relatively small. This might be consistent with the hypothesis that an endolymphatic hydrops is a marker of disordered inner ear homeostasis rather than the cause of the clinical symptoms of Ménière's disease.
Objective:
Assessment of cochlear function during induction of a destructive acute endolymphatic hydrops.
Materials And Methods:
During repetitive microinjections of 0.5 microl of artificial endolymph at a rate of 50 nl/s the 2f1-f2 and f2-f1 cochlear microphonics distortion products (CMDP) and 2f1-f2 distortion products otoacoustic emissions (DPOAE) were recorded in the guinea pig.
Results:
A 'catastrophe' occurred in the inner ear when 2.5-3.5 microl of artificial endolymph was injected. A rupture of Reissner's membrane was then found, most often in the apical turn of the cochlea. This rupture had only minor effects on the endocochlear potential, whereas it caused a marked decrease in 2f1-f2 DPOAE amplitude. The 2f1-f2 and f2-f1 CMDP amplitude increased during each injection prior to the rupture. After the rupture the f2-f1 CMDP amplitude decreased during each injection, possibly due to a shift of the cochlear transducer operating point position.
