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Fishmeal extract bile salt lactose agar--a differential medium for enteric bacteria
K Subbannayya1, J Udayalaxmi, M Anugraha
1Department of Microbiology, Kasturba Medical College, Mangalore, India. dr_s_kotigadde@yahoo.co.in
Indian Journal of Experimental Biology
|August 24, 2006
Summary
A new medium, fishmeal extract bile salt lactose agar (FEBLA), effectively differentiates enteric bacteria. This cost-effective FEBLA is a viable alternative for preliminary bacterial identification.
Area of Science:
- Microbiology
- Bacteriology
- Clinical Diagnostics
Background:
- Accurate differentiation of enteric bacteria is crucial for clinical diagnostics.
- Existing differential media may have limitations in contrast or cell morphology observation.
Purpose of the Study:
- To develop and evaluate a novel differential medium, fishmeal extract bile salt lactose agar (FEBLA).
- To compare FEBLA's performance against MacConkey's agar for differentiating lactose fermenters (LF) from non-lactose fermenters (NLF).
Main Methods:
- FEBLA medium was formulated and tested for bacterial growth and differentiation.
- Comparative analysis with MacConkey's agar was performed.
- Cell morphology changes were observed using microscopy.
Main Results:
- FEBLA demonstrated performance on par with MacConkey's agar.
- FEBLA provided a pronounced contrast between LF and NLF colonies.
- Klebsiella pneumoniae exhibited enhanced mucoid colony production and altered cell morphology (longer, thicker cells with capsular material) on FEBLA compared to MacConkey's agar.
Conclusions:
- FEBLA is a simple, cost-effective differential medium.
- It offers distinct advantages in visualizing colony morphology and bacterial cell characteristics.
- FEBLA is a suitable alternative for the preliminary identification of enteric bacteria.
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