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Published on: September 11, 2020
[Molecular characterization of Colombian invasive serotype 5 Streptococcus pneumoniae isolates recovered between 1994
Carolina Firacative1, Jaime Moreno, Elizabeth Castañeda
1Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, D.C., Colombia.
Background:
Streptococcus pneumoniae serotype 5 is an important cause of invasive disease in Colombia where the circulation of the clone Colombia5-19, penicillin susceptible but tetracycline and chloramphenicol resistant, has been demonstrated.
Objective:
To establish the genetic relatedness among Colombian invasive S. pneumoniae serotype 5 isolates, recovered between 1994 and 2004, and the clone Colombia(5)-19.
Materials And Methods:
Eighty three isolates with penicillin, vancomycin, ceftriaxone, erythromycin, trimethoprim-sulfametoxazole, chloramphenicol and tetracycline susceptibility patterns were studied. Of these isolates, 29 were recovered from children less than 5 years of age. DNA restriction patterns of all isolates were determined for pulsed field gel electrophoresis, using the Smal enzyme. Genetic similarity among isolates and the clone Colombia(5)-19 was established according to Tenover's criteria and the Fingerprintingä II program.
Results:
All isolates were related with the Colombia(5)-19 clone and belonged to the electrophoretic pattern A, of which 17 subtypes were derived. Most of the isolates belonged to patterns A (38,6%), A8 (21,7%) and A5 (12%), the other 23 isolates belonged to 15 electrophoretic patterns. Thirty -four isolates resistant both to tetracycline and chloramphenicol were related with electrophoretic patterns A (n = 32), A16 (n = 1) and A28 (n = 1), which have a band of 340 Kb in common with the clone.
Conclusion:
These results showed the continuing circulation of S pneumoniae serotype 5 isolates in the country, and which are genetically related with the clone Colombia(5)-19.
Insights
Streptococcus pneumoniae serotype 5 isolates in Colombia are genetically linked to the Colombia5-19 clone. This study confirms the ongoing circulation of these invasive strains, highlighting their antibiotic resistance patterns.
Area of Science:
- Microbiology
- Genetics
- Epidemiology
Context:
- Streptococcus pneumoniae serotype 5 causes invasive disease in Colombia.
- The Colombia5-19 clone is noted for its resistance to tetracycline and chloramphenicol.
- Previous studies indicated the circulation of this specific clone.
Purpose:
- To determine the genetic relatedness of invasive Streptococcus pneumoniae serotype 5 isolates from Colombia.
- To compare these isolates with the known Colombia5-19 clone.
- To analyze genetic similarities and antimicrobial resistance profiles.
Summary:
- Eighty-three Streptococcus pneumoniae serotype 5 isolates were analyzed using pulsed-field gel electrophoresis.
- All isolates showed genetic relatedness to the Colombia5-19 clone, belonging to electrophoretic pattern A.
- Thirty-four isolates resistant to tetracycline and chloramphenicol were identified and linked to specific subtypes of pattern A.
Impact:
- Confirms the sustained circulation of Streptococcus pneumoniae serotype 5 in Colombia.
- Demonstrates the genetic linkage of invasive isolates to the Colombia5-19 clone.
- Provides insights into the molecular epidemiology and antibiotic resistance of this pathogen in the region.
