Related Experiment Video
Updated: Jul 20, 2026
10:33
Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
Published on: January 8, 2014
Isolation and characterization of full-length recombinant cattle PrPC protein
S L Kal'nov1, V B Grigor'ev, K P Alekseev
1D I Ivanovsky Institute of Virology, Russian Academy of Medical Sciences. kalnov@narvac.com
Bulletin of Experimental Biology and Medicine
|August 26, 2006
Abstract:
Full-length Bos taurus PrPC protein was obtained in the eu- and prokaryotic expression systems. Immunoblotting and indirect enzyme immunoassay demonstrated high specificity and antigenic activity of full-length proteins in the reactions with monoclonal antibodies (anti-SAF-32 and VRQ-84). Membrane location of recombinant PrPC protein in insect cells was shown by immunofluorescent analysis.
