Related Experiment Video
Updated: Jul 20, 2026

Generic Protocol for Optimization of Heterologous Protein Production Using Automated Microbioreactor Technology
Published on: December 15, 2017
Substrate induction and statistical optimization for the production of chitosanase from Microbacterium sp. OU01
Yuying Sun1, Baoqin Han, Wanshun Liu
1College of Marine Life Sciences, Ocean University of China, Qingdao 266003, PR China.
Abstract:
The chitosanase production was markedly enhanced by substrate induction, statistical optimization of medium composition and culture conditions by Microbacterium sp. OU01 in shake-flask. A significant influence of (NH(4))(2)SO(4), MgSO(4).7H(2)O and initial pH on chitosanase production was noted with Plackett-Burman design. It was then revealed with the method of steepest ascent and response surface methodology (RSM) that 19.0g/L (NH(4))(2)SO(4), 1.3g/L MgSO(4) and an initial pH of 2.0 were optimum for the production of chitosanase; colloidal chitosan appeared to be the best inducer for chitosanase production by Microbacterium sp. OU01. This optimization strategy led to the enhancement of chitosanase from 3.6U/mL to 118U/mL.
Related Concept Videos
Bioreactor Controls-III
Upstream Processing
Production of Organic Acids
Production of Antibiotics
Production of Alcohol
iChip
