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Updated: Jul 20, 2026
11:37
Analyzing Protein Dynamics Using Hydrogen Exchange Mass Spectrometry
Published on: November 29, 2013
Semi-automated data processing of hydrogen exchange mass spectra using HX-Express
David D Weis1, John R Engen, Ignatius J Kass
1Department of Chemistry, University of New Mexico, Albuquerque, New Mexico, USA.
Journal of the American Society for Mass Spectrometry
|August 26, 2006
Summary
HX-Express is a new Microsoft Excel tool that speeds up hydrogen exchange mass spectrometry (HX-MS) data analysis. It helps researchers quickly generate deuterium uptake and peak width plots for better quality control.
Area of Science:
- Biochemistry
- Analytical Chemistry
- Mass Spectrometry
Background:
- Hydrogen exchange mass spectrometry (HX-MS) is a powerful technique for studying protein structure and dynamics.
- Manual analysis of HX-MS data can be time-consuming and labor-intensive.
- Accelerating data analysis is crucial for efficient research workflows.
Purpose of the Study:
- To introduce HX-Express, a Microsoft Excel utility designed to accelerate HX-MS data analysis.
- To provide a semi-automated solution for generating deuterium uptake and peak width plots.
- To ensure the accuracy and quality control of HX-MS data analysis.
Main Methods:
- Development of a Microsoft Excel utility, HX-Express.
- Semi-automated data analysis workflow requiring user identification of peaks.
- Import functionality for m/z versus intensity data lists or direct import from Waters MassLynx software.
Main Results:
- HX-Express significantly accelerates the analysis of HX-MS data compared to manual processing.
- The utility generates deuterium uptake and peak width plots.
- Analysis results are consistent with manual data processing.
- Visual and numeric displays are provided for quality control.
Conclusions:
- HX-Express offers a substantial speed improvement for HX-MS data analysis.
- The semi-automated approach balances efficiency with user control.
- The tool enhances quality control through preserved visual and numeric displays.
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