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Updated: Jul 20, 2026

Laser Micro-Irradiation to Study DNA Recruitment During S Phase
Published on: April 16, 2021
One ring to rule them all? Another cellular responsibility for PCNA
1Genome Damage and Stability Centre, University of Sussex, Brighton, BN19RQ, UK. c.m.green@sussex.ac.uk
Abstract:
To prevent duplication or loss of genomic regions during DNA replication, it is essential that the entire genome is copied precisely once every S phase. Cells achieve this by mutually exclusive regulation of origin firing and licensing. A crucial protein that is involved in origin licensing is chromatin licensing and DNA replication factor 1 (CDT1) and, therefore, activity of this protein must be strictly controlled. Four recent articles have demonstrated that proliferating cell nuclear antigen (PCNA), an essential sliding clamp used in replication and DNA repair, has a crucial role in this process by mediating the proteasomal degradation of CDT1.
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