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Related Concept Videos

Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
The Contractile Ring02:15

The Contractile Ring

Contractile rings are composed of microfilaments and are responsible for separating the daughter cells during cytokinesis. Contractile ring assembly proceeds along with other cell cycle events; however, very few mechanistic details are known about the timing and coordination of the contractile rings with the cell cycle.
A small GTPase, RhoA, controls the function and assembly of the contractile ring. RhoA belongs to the Ras superfamily of proteins. The activation of formins by RhoA promotes...
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
Cell-mediated Immune Responses01:40

Cell-mediated Immune Responses

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The Cell Cycle Control System01:28

The Cell Cycle Control System

The cell cycle regulation directs how a cell proceeds from one phase to the next and begins mitosis. The cell cycle control system includes intracellular regulatory molecules and external triggers. They provide "stop" or "advance" signals and operate at specific cell cycle stages termed checkpoints to ensure that a particular process is completed before the cell advances to the next phase.
Cyclins and cyclin-dependent kinases (Cdks) are the primary cell cycle regulators and function at the cell...
The Cell Cycle Control System02:11

The Cell Cycle Control System

The cell cycle is an organized set of events that leads the cell to divide into two daughter cells, each containing chromosomes identical to the parent cell. It is the cell cycle that leads to the formation of an entire organism from a single-cell zygote. Besides, cell division also functions in the renewal or repair of tissues in adult multicellular eukaryotes. For example, in the bone marrow, the stem cells divide to form new blood cells. Although essential for several functions, cell...

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Related Experiment Video

Updated: Jul 20, 2026

Laser Micro-Irradiation to Study DNA Recruitment During S Phase
07:11

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Published on: April 16, 2021

One ring to rule them all? Another cellular responsibility for PCNA.

Catherine M Green1

  • 1Genome Damage and Stability Centre, University of Sussex, Brighton, BN19RQ, UK. c.m.green@sussex.ac.uk

Trends in Molecular Medicine
|August 26, 2006
PubMed
Summary

Cellular DNA replication requires precise genome copying each S phase. Proliferating cell nuclear antigen (PCNA) controls CDT1 protein levels, preventing DNA replication errors.

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Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

Related Experiment Videos

Last Updated: Jul 20, 2026

Laser Micro-Irradiation to Study DNA Recruitment During S Phase
07:11

Laser Micro-Irradiation to Study DNA Recruitment During S Phase

Published on: April 16, 2021

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging
06:44

Assessment of Global DNA Double-Strand End Resection using BrdU-DNA Labeling coupled with Cell Cycle Discrimination Imaging

Published on: April 28, 2021

Area of Science:

  • Molecular Biology
  • Cell Biology
  • Genetics

Background:

  • Accurate DNA replication ensures genome stability by copying the entire genome exactly once per cell cycle.
  • Regulation of DNA replication involves controlling origin firing and licensing to prevent re-replication or loss of genomic regions.
  • Chromatin licensing and DNA replication factor 1 (CDT1) is critical for DNA replication origin licensing and its activity must be tightly regulated.

Purpose of the Study:

  • To investigate the role of proliferating cell nuclear antigen (PCNA) in regulating CDT1 activity.
  • To elucidate the mechanism by which PCNA controls CDT1 during DNA replication.

Main Methods:

  • The study likely involved molecular biology techniques to analyze protein interactions and degradation pathways.
  • Experiments may have focused on the proteasomal degradation of CDT1 mediated by PCNA.

Main Results:

  • Four recent studies demonstrate that proliferating cell nuclear antigen (PCNA) plays a key role in regulating CDT1.
  • PCNA mediates the proteasomal degradation of CDT1, thereby controlling its activity.

Conclusions:

  • PCNA is essential for preventing DNA replication errors by regulating CDT1 degradation.
  • This mechanism ensures the precise, once-per-cell-cycle replication of the genome.