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Native-like SARS-CoV-2 Spike Glycoprotein Expressed by ChAdOx1 nCoV-19/AZD1222 Vaccine
Yasunori Watanabe1,2, Luiza Mendonça3, Elizabeth R Allen4
1School of Biological Sciences, University of Southampton, Southampton, SO17 1BJ, U.K.
ACS Central Science
|May 31, 2021
Summary
The ChAdOx1 nCoV-19/AZD1222 vaccine demonstrates native-like spike protein processing and assembly. This confirms adenovirus vectors are a leading platform for effective SARS-CoV-2 vaccines.
Area of Science:
- Vaccinology
- Structural Biology
- Virology
Background:
- SARS-CoV-2 vaccine development targets the spike (S) glycoprotein.
- Adenovirus-vectored vaccines require proper antigen processing for neutralizing antibody generation.
Purpose of the Study:
- To characterize the structure, conformation, and glycosylation of the S protein from the ChAdOx1 nCoV-19/AZD1222 vaccine.
- To confirm the suitability of adenovirus vectors for SARS-CoV-2 vaccine delivery.
Main Methods:
- Structural and biochemical analysis of the S protein.
- Assessment of post-translational processing and cellular expression.
Main Results:
- The ChAdOx1 vaccine's S protein exhibits native-like post-translational processing and assembly.
- Expressed S proteins adopt the trimeric prefusion conformation on cell surfaces.
Conclusions:
- Adenovirus-vectored vaccines like ChAdOx1 nCoV-19/AZD1222 effectively present SARS-CoV-2 spike protein.
- These findings support adenovirus vectors as a promising platform for COVID-19 vaccines.
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