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Accessing the Cytotoxicity and Cell Response to Biomaterials
Published on: July 8, 2021
Tissue and cell reactions to implanted root-end filling materials.
Chia-Tze Kao1, Chung-Hung Tsai, Tsui-Hsien Huang
1Institute of Medicine, Chung Shan Medical University.
Journal of Materials Science. Materials in Medicine
|August 26, 2006
Summary
Mineral trioxide aggregate (MTA) showed superior biocompatibility compared to calcium hydroxide and eugenol-based materials in vitro and in vivo. MTA demonstrated better cell survival and tissue integration for root-end filling applications.
Area of Science:
- Biomaterials Science
- Endodontics
- Regenerative Dentistry
Background:
- Root-end filling materials are crucial for periradicular tissue repair.
- Optimal materials require excellent cell and tissue compatibility.
- Evaluating biocompatibility and tissue response is essential for clinical success.
Purpose of the Study:
- To compare the in vitro biocompatibility and in vivo tissue reaction of three root-end filling materials.
- Materials evaluated included calcium hydroxide-based, eugenol-based, and mineral trioxide aggregate (MTA).
Main Methods:
- In vitro: Human osteosarcoma cell line exposed to immersed root-end filling materials.
- In vivo: Materials implanted in rats, with observations at 6 and 8 weeks.
- Histopathological and radiographic analyses were performed.
Main Results:
- Mineral trioxide aggregate (MTA) exhibited the highest in vitro cell survival rate (p<0.05).
- In vivo, MTA showed good tissue tolerance.
- Calcium hydroxide implants developed a radiopaque ring; eugenol-based materials elicited inflammatory responses.
Conclusions:
- Mineral trioxide aggregate (MTA) demonstrates superior biocompatibility and tissue integration compared to calcium hydroxide and eugenol-based materials.
- MTA is a well-tolerated root-end filling material, supporting its clinical use.
- Further research may explore MTA's regenerative potential in periradicular healing.
