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Monoclonal antibodies to barley alpha-amylase I: production, characterization, and application to a specific
D Lecommandeur1, F Merlin, J Daussant
1Laboratoire de Physiologie des Organes Végétaux, C.N.R.S., Meudon, France.
Monoclonal antibodies (MAbs) to barley alpha-amylase I have been produced, purified and characterized. Six are specific for the alpha-amylase I isoform, while one also reacts with alpha-amylase II. All but one recognize the antigen in solution. Topology of the MAbs was investigated by additivity in ELISA, which led us to subdivide them into four groups. An immunoassay was developed using one of these MAbs, to measure alpha-amylase I activity in an extract containing both isoforms.
Monoclonal antibodies (MAbs) to barley alpha-amylase I have been produced, purified and characterized. Six are specific for the alpha-amylase I isoform, while one also reacts with alpha-amylase II. All but one recognize the antigen in solution. Topology of the MAbs was investigated by additivity in ELISA, which led us to subdivide them into four groups. An immunoassay was developed using one of these MAbs, to measure alpha-amylase I activity in an extract containing both isoforms.