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[Study on immobilization of Acidithiobacillus ferrooxidans using PVA-Ca(NO3)2 method]
1College of Life Science, Lanzhou University, Lanzhou 730000, China. open2000cn@163.com
Wei Sheng Wu Xue Bao = Acta Microbiologica Sinica
|August 29, 2006
Summary
A novel cell immobilization method using PVA and sodium alginate offers a simple, cost-effective, and scalable solution for entrapping Acidithiobacillus ferrooxidans. This technique enhances bead stability and cell activity for industrial applications.
Area of Science:
- Biotechnology
- Bioprocess Engineering
- Microbial Immobilization
Background:
- Cell immobilization is crucial for enhancing microbial stability and activity in bioprocesses.
- Traditional methods often face challenges like cell aggregation and toxicity of immobilization agents.
Purpose of the Study:
- To develop a novel and efficient cell immobilization technique for Acidithiobacillus ferrooxidans.
- To evaluate the performance and practicality of the new immobilization method for industrial applications.
Main Methods:
- Utilizing a complex of polyvinyl alcohol (PVA) and sodium alginate as the entrapment medium.
- Extruding the mixture into calcium nitrate solution to form beads, followed by freezing and thawing cycles.
- Assessing the physical properties and oxidation activity of the immobilized cells.
Main Results:
- Achieved a maximum oxidation rate of 2.45g Fe2+ /(L x h) using immobilized Acidithiobacillus ferrooxidans.
- The new method effectively prevents PVA bead agglomeration and eliminates biological toxicity.
- Immobilized beads exhibited superior mechanical strength and oxidation activity compared to other methods.
Conclusions:
- The developed PVA-sodium alginate immobilization technique is simple, cost-effective, and uses low-toxicity reagents.
- This method offers improved bead characteristics and high cell activity, making it highly practicable for industrial-scale applications.
