Related Experiment Video
Updated: Jul 20, 2026
08:26
Development of Amelogenin-chitosan Hydrogel for In Vitro Enamel Regrowth with a Dense Interface
Published on: July 10, 2014
Self-etching adhesives increase collagenolytic activity in radicular dentin.
Franklin R Tay1, David H Pashley, Robert J Loushine
1Department of Oral Biology and Maxillofacial Pathology, Medical College of Georgia, Augusta, GA 30912-1129, USA.
Journal of Endodontics
|August 29, 2006
Summary
Mild self-etching dental adhesives activate latent matrix metalloproteinases (MMPs) in root dentin. This enzyme activation, releasing collagenolytic activity, may compromise the long-term stability of bonded restorations in root canals.
Area of Science:
- Endodontics
- Dental Materials Science
- Biochemistry
Background:
- Endogenous matrix metalloproteinases (MMPs) in dentin can degrade hybrid layers.
- Activation of these MMPs is a concern for the longevity of dental adhesive bonds.
Purpose of the Study:
- To investigate if instrumented intraradicular dentin has latent collagenolytic activity.
- To determine if mild self-etching adhesives activate this activity.
Main Methods:
- Root dentin powder was obtained from cleaned and shaped canals.
- Dentin was treated with MMP inhibitors (chlorhexidine, EDTA) or self-etching adhesives.
- Collagenolytic activity was measured using a fluorometer.
- Demineralization was assessed via Transmission Electron Microscopy (TEM).
Main Results:
- Instrumented dentin showed low collagenolytic activity, inhibited by chlorhexidine and EDTA.
- Self-etching adhesives partially demineralized dentin and activated latent MMPs, increasing activity 14-15 fold.
- Chlorhexidine incompletely inactivated the activated MMPs.
Conclusions:
- Mild self-etching adhesives activate latent MMPs in root dentin without denaturation.
- This activation may negatively impact the durability of bonded root canal fillings and posts.
