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Human alveolar bone cells interact with ProRoot and tooth-colored MTA
Ebtehal Al-Rabeah1, Hiran Perinpanayagam, Don MacFarland
1Department of Oral Biology, School of Dental Medicine, University at Buffalo, Buffalo, NY 14214-8031, USA.
Journal of Endodontics
|August 29, 2006
Summary
Mineral trioxide aggregate (MTA) supports human alveolar bone cell attachment and proliferation. Both gray and tooth-colored MTA formulations effectively promote cell growth and matrix formation for tissue regeneration.
Area of Science:
- Biomaterials Science
- Regenerative Dentistry
- Cell Biology
Background:
- Mineral trioxide aggregate (MTA) is crucial for periradicular tissue repair and regeneration.
- Understanding cellular responses to MTA is vital for optimizing its clinical applications.
Purpose of the Study:
- To investigate the response of human alveolar bone cells to different types of MTA.
- To evaluate the biocompatibility and regenerative potential of MTA materials.
Main Methods:
- Human alveolar bone explant cultures were established.
- Early passage cells were seeded onto ProRoot (gray) MTA, tooth-colored MTA, and MTA with local anesthetic.
- Scanning electron microscopy was used to assess cell attachment, spreading, and matrix formation over 14 days.
Main Results:
- Human alveolar bone cells readily attached and spread onto all tested MTA formulations within 24 hours.
- Significant cell proliferation and matrix-like layer formation were observed within 7 days.
- Comparable cell attachment and surface interactions were noted for gray MTA, white MTA, and MTA with anesthetic over 14 days.
Conclusions:
- Surgically derived human alveolar bone cells offer a clinically relevant model for MTA evaluation.
- Both ProRoot and tooth-colored MTA demonstrate excellent capacity to support cell attachment, proliferation, and matrix formation.
- MTA formulations are effective in promoting cellular responses necessary for periradicular tissue regeneration.
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