Temperature as a variable in liquid chromatography: development and application of a model for the separation of
A M Edge1, S Shillingford, C Smith
1Department of Drug Metabolism and Pharmacokinetics, AstraZeneca Pharmaceuticals, Alderley Park, Macclesfield, Cheshire SK10 4TG, UK. anthonye@astrazeneca.com <anthonye@astrazeneca.com>
Abstract:
The use of high temperatures in liquid chromatography allows for the use of a purely aqueous mobile phase. At elevated temperatures water possesses many of the characteristics of organic solvents in terms of eluotropic strength, as well as having a lower viscosity. A model is developed, based on data obtained using a range of model drugs, which demonstrates the relationship between temperature, flow and pressure. Experimental data from different column types, at temperatures from 40 degrees C to 180 degrees C, is presented which matches well with the predicted data from the model.
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