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Updated: Jun 25, 2026

DamID-seq: Genome-wide Mapping of Protein-DNA Interactions by High Throughput Sequencing of Adenine-methylated DNA Fragments
Published on: January 28, 2016
Giant proteins that move DNA: bullies of the genomic playground
Nicholas R Cozzarelli1, Gregory J Cost, Marcelo Nöllmann
1Department of Molecular and Cell Biology, University of California at Berkeley, Berkeley, California 94720-3204, USA.
Abstract:
As genetic material DNA is wonderful, but as a macromolecule it is unruly, voluminous and fragile. Without the action of DNA replicases, topoisomerases, helicases, translocases and recombinases, the genome would collapse into a topologically entangled random coil that would be useless to the cell. We discuss the organization, movement and energetics of these proteins that are crucial to the preservation of a molecule that has such beautiful biological but challenging physical properties.
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