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Updated: May 20, 2025

Dissection of Enhancer Function Using Multiplex CRISPR-based Enhancer Interference in Cell Lines
Published on: June 2, 2018
Redefining enhancer action: Insights from structural, genomic, and single-molecule perspectives
David Llères1, Andres Cardozo Gizzi2, Marcelo Nollmann1
1Centre de Biologie Structurale, Univ Montpellier, CNRS UMR 5048, INSERM U1054, 34090 Montpellier, France.
Abstract:
This review explores recent emerging insights into enhancer action, focusing on underexplored aspects such as the physical size of regulatory elements, the stochasticity of transcription factor binding and chromatin structure, and the role of nonlinear processes in reconciling longstanding discrepancies between theoretical models and experimental observations. Together, these insights provide a nuanced view of enhancer biology, highlighting the complexity of gene regulation and the need for innovative methodologies to further decode enhancer mechanisms.
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