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Published on: May 6, 2015
[Expression of anti-HBc single-chain variable fragment mediated by recombinant replication defective adenovirus in
Zheng-hao Tang1, Yong-sheng Yu, Hui-hui Ma
1Department of Infectious Diseases, Shanghai Jiaotong University Affiliated Sixth People's Hospital, Shanghai 200233, China. tzhhao@hotmail.com
Objectives:
To define the expression of single-chain variable fragment (ScFv) against hepatitis B virus core protein (HBc) mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene in vitro and to define the activity of anti-HBc ScFv combining HBcAg.
Methods:
The recombinant adenoviruses carrying anti-HBc ScFv gene generated by homologous recombination in bacteria and packaged in 293 cells were transfected into HepG2 cells, and the anti-HBc ScFv was detected using SDS-PAGE and Western blot.
Results:
Green fluorescent protein (GFP) was observed in HepG2 cells after the transfection. SDS-PAGE displayed a protein strap about 2.7 x 10(4), and the result of Western blot displayed a positive reactive strap.
Conclusion:
Anti-HBc ScFv can be expressed in cells mediated by recombinant replication defective adenovirus carrying the anti-HBc ScFv gene.
