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Pros and cons of immunochemical and enzymatic method in the diagnosis of Wilson's disease
J V Gnanou1, V G Thykadavil, V Thuppil
1University of Seychelles American Institute of Medicine, MAHE, Seychelles. gnanou_j@yahoo.com
Background:
Immunochemical method of measuring Ceruloplasmin (Cp) levels for the diagnosis of Wilson's disease has replaced enzymatic method for the main reason of being more sensitive and quantitative.
Settings And Design:
In this study, we compared both the methods for various factors such as sensitivity, specificity and the time consumed in the diagnosis of Wilson's disease.
Materials And Methods:
Serum samples from patients (n=33) with a provisional diagnosis of Wilson's disease were analyzed for Cp levels by enzymatic copper oxidase method and immunochemical method using polyclonal antibodies specific to Cp embedded in agar.
Statistical Analysis:
Pearson's regression analysis was performed to compare the two methods.
Results:
The mean Cp obtained by immunochemical method is 5.87 mm +/- 1.17 and by enzymatic method, it is 0.37 (Optical Density) +/- 0.136. Pearson's Regression analysis of the measurements showed a good correlation with an 'r' value of 0.765 between the two methods.
Conclusion:
A good correlation indicated that these two tests are comparable and thus both these methods can be used together for a definitive and better diagnosis of Wilson's disease.
