Related Experiment Video
Updated: Jul 20, 2026

Preparation of Fungal and Plant Materials for Structural Elucidation Using Dynamic Nuclear Polarization Solid-State NMR
Published on: February 12, 2019
[Structure of fungal cell wall polysaccharides]
Nobuyuki Shibata1, Yoshio Okawa
1Department of Infection and Host Defense, Tohoku Pharmaceutical University, Miyagi, Japan.
Abstract:
Candida albicans mannan consists of the alpha-1,6-linked backbone moiety and the alpha-1,2- and alpha-1,3-linked side chains. It also contains alpha-1,6-branched mannose units, beta-1,2-linked mannose units, and phosphate groups. The cell wall mannans of the genus Candida possess three types of beta-1,2 linked mannose units. One is linked via the phosphodiester linkage, the second type is connected to an alpha-1,2-linked mannose unit, and the third type is attached to an alpha-1,3-linked mannose unit. These beta-1,2-linked mannose units showed a strong antigenicity and produce the characteristic NMR chemical shifts. Using two-dimensional NMR techniques, we will practically determine the structure of these polysaccharides in a nondestructive manner.
Related Concept Videos
Cellulose and Pectic Polysaccharides
As a cell matures, its cell wall specializes according to its type. For example, the parenchyma cells of...
Overview of Fungi
Chemistry of Carbohydrates
Plant Cell Wall
Plant Cell Wall
Fungal Group Zygomycota

