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CD44 is the principal cell surface receptor for hyaluronate
A Aruffo1, I Stamenkovic, M Melnick
1Department of Genetics, Harvard Medical School, Boston, Massachusetts 02115.
Cell
|June 29, 1990
Summary
CD44 cell surface protein binds to hyaluronate, a key component of the extracellular matrix. This interaction is crucial for cell attachment and is sensitive to hyaluronidase digestion.
Area of Science:
- Immunology
- Cell Biology
- Glycobiology
Background:
- CD44 is a cell surface glycoprotein involved in cell adhesion.
- Its role in mediating attachment to extracellular matrix components is well-established.
Purpose of the Study:
- To investigate the interaction of CD44 with hyaluronate.
- To characterize the binding properties of soluble CD44-immunoglobulin fusion proteins.
Main Methods:
- Creation and characterization of soluble CD44-immunoglobulin fusion proteins.
- Assays using tissue sections and primary cultured high endothelial cells.
- Enzymatic digestion with hyaluronidase and inhibition studies with glycosaminoglycans.
Main Results:
- Soluble CD44 fusion proteins bind to tissue sections and lymph node high endothelial cells.
- CD44 binding is sensitive to hyaluronidase, indicating hyaluronate as a ligand.
- Binding is inhibited by hyaluronate and chondroitin sulfates.
Conclusions:
- CD44 functions as a receptor for hyaluronate on endothelial cells.
- This interaction is important for cell adhesion in various tissues, including lymph nodes.
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