Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jul 5, 2026

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

Silanizing glassware.

¹Massachusetts General Hospital and Harvard Medical School, Boston, Massachusetts, USA.

Current Protocols in Immunology

|April 25, 2008

Summary

This summary is machine-generated.

Silanizing glassware prevents solute adsorption, crucial for sensitive biomolecules like nucleic acids and proteins. This protocol details treating lab glassware to enhance its properties.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Selective activation of nuclear factor kappa B in the cochlea by sensory and inflammatory stress.

Neuroscience·2009

Same author

Silanizing glassware.

Current protocols in protein science·2008

Same author

Silanizing glassware.

Current protocols in molecular biology·2008

Same author

Silanizing glassware.

Current protocols in cell biology·2008

Same author

Efficient and stable retroviral transfection of ovine endothelial cells with green fluorescent protein for cardiovascular tissue engineering.

Tissue engineering·2003

Same author

Targeted disruption of the murine Fanconi anemia gene, Fancg/Xrcc9.

Blood·2001

Same journal

A Comprehensive Experimental Guide to Studying Cross-Presentation in Dendritic Cells In Vitro.

Current protocols in immunology·2020

Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020

Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020

Same journal

Immunologic Studies in Humans.

Current protocols in immunology·2020

Same journal

Monoclonal Antibodies to Human Cell Surface Antigens.

Current protocols in immunology·2020

Same journal

Protocols for Experimental Sjögren's Syndrome.

Current protocols in immunology·2020

See all related articles

Area of Science:

Biochemistry
Materials Science
Laboratory Techniques

Background:

Glass surfaces can adsorb solutes, affecting experimental accuracy.
Hydrophobicity of glassware is critical for certain biological samples.
Low concentrations of sticky solutes like nucleic acids and proteins are prone to adsorption.

Purpose of the Study:

To provide a protocol for silanizing (siliconizing) laboratory glassware.
To outline methods for preventing solute adsorption to glass surfaces.
To enhance the hydrophobicity of glassware for specific applications.

Main Methods:

Detailed protocol for glassware silanization.
Application of silanizing agents to glass surfaces.
Washing and drying procedures for treated glassware.

More Related Videos

Self-Assembly of Microtubule Tactoids

Self-Assembly of Microtubule Tactoids

Published on: June 23, 2022

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Related Experiment Videos

Last Updated: Jul 5, 2026

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Preparation of Segmented Microtubules to Study Motions Driven by the Disassembling Microtubule Ends

Published on: March 15, 2014

Self-Assembly of Microtubule Tactoids

Self-Assembly of Microtubule Tactoids

Published on: June 23, 2022

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Imaging Molecular Adhesion in Cell Rolling by Adhesion Footprint Assay

Published on: September 27, 2021

Main Results:

Effectively reduced adsorption of "sticky" solutes.
Increased hydrophobicity of treated glassware.
Protocol ensures consistent and reliable results.

Conclusions:

Silanization is an effective method for preparing glassware.
Treated glassware is essential for working with sensitive biomolecules.
This protocol offers a reliable approach for glassware treatment.