Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

PI3K/mTOR/AKT Signaling Pathway

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1 (mTORC1) and mTOR complex 2 (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast, mTORC2 consists of a rapamycin-insensitive companion...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

DNA Damage can Stall the Cell Cycle

DNA Damage can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

DNA Damage Can Stall the Cell Cycle

DNA Damage Can Stall the Cell Cycle

In response to DNA damage, cells can pause the cell cycle to assess and repair the breaks. However, the cell must check the DNA at certain critical stages during the cell cycle. If the cell cycle pauses before DNA replication, the cells will contain twice the amount of DNA. On the other hand, if cells arrest after DNA replication but before mitosis, they will contain four times the normal amount of DNA. With a host of specialized proteins at their disposal,cells must use the right protein at...

Interactions Between Signaling Pathways

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...

Amplifying Signals via Enzymatic Cascade

Amplifying Signals via Enzymatic Cascade

When a ligand binds to a cell-surface receptor, the receptor's intracellular domain changes shape, which may either activate its enzyme function or allow its binding to other molecules. The initial signal is amplified by most signal transduction pathways. This means that a single ligand molecule can activate multiple molecules of a downstream target. Proteins that relay a signal are most commonly phosphorylated at one or more sites, activating or inactivating the protein. Kinases catalyze the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Dose and LET-dependence of gastric cancer risk in Apc<sup>1638N/+</sup> mice exposed to space-relevant radiation types.

Life sciences in space research·2026

Same author

Lipidomics of longer-term effects in the murine model after exposure to mixed field radiation and combined infection.

Metabolomics : Official journal of the Metabolomic Society·2026

Same author

A cross-talk between p16High senescence and cellular reprogramming.

Clinical science (London, England : 1979)·2026

Same author

Polymeric sorbent phase sorptive extraction for radiation metabolomics.

RSC advances·2026

Same author

The circadian gene Dec2 promotes pancreatic cancer progression and dormancy through immune evasion.

Developmental cell·2026

Same author

High-linear energy transfer radiation disrupts natural killer cell surveillance of senescent intestinal cells in the mouse intestine.

Molecular biomedicine·2026

Search research articles

Related Experiment Video

Updated: Jul 20, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Wip1 phosphatase modulates ATM-dependent signaling pathways.

Sathyavageeswaran Shreeram¹, Oleg N Demidov, Weng Kee Hee

¹Institute of Molecular and Cell Biology, 61 Bioplis Drive, Proteos, 138673, Singapore.

|September 5, 2006

Summary

Deleting the Wip1 phosphatase gene prevents tumor development by activating ATM kinase. Wip1 normally deactivates ATM, a key DNA repair protein, after DNA damage.

More Related Videos

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Published on: September 6, 2024

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Related Experiment Videos

Last Updated: Jul 20, 2026

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Development and Application of Rapamycin-regulated Tyrosine Phosphatases

Published on: September 6, 2024

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Detection and Visualization of DNA Damage-induced Protein Complexes in Suspension Cell Cultures Using the Proximity Ligation Assay

Published on: June 9, 2017

Area of Science:

Molecular Biology
Biochemistry
Cancer Research

Background:

The Wip1 phosphatase, encoded by the Ppm1d gene, plays a role in cellular transformation and tumor development.
ATM kinase is a critical regulator of DNA damage response pathways.

Purpose of the Study:

To investigate the role of Wip1 phosphatase in the activation and regulation of ATM kinase signaling.
To elucidate the mechanism by which Wip1 influences DNA damage response.

Main Methods:

Gene deletion and overexpression studies in cellular and mouse models.
Analysis of ATM kinase activation and phosphorylation status.
Assessment of DNA damage repair signaling pathways.

Main Results:

Deficiency of Wip1 phosphatase led to constitutive activation of ATM kinase.
Overexpression of Wip1 suppressed ATM activation following DNA damage.
Wip1 directly dephosphorylated ATM at Ser1981, a key site for its activation.
Wip1 is crucial for resetting ATM phosphorylation during DNA repair.

Conclusions:

Wip1 phosphatase acts as a negative regulator of ATM kinase activity.
Wip1 is an integral component of the ATM-dependent DNA damage signaling pathway.
Targeting Wip1 may offer therapeutic strategies for cancer treatment.