[Brush cytology for the evaluation of dry-eye]

A special brush was developed for collection of conjunctival cells with better integrity of the harvested specimens. Using this brush, cells were collected from temporal bulbar conjunctiva of 20 normal eyes and 40 eyes with dry-eye, and were stained by the modified Papanicolau method, Keratinized cells (K) and goblet cells (G) were classified into 5 categories, from (-) to ( ) according to the numbers of the cells observed. Normal eyes were categorized as K (-):16, K(+/-):4, G (+):2, G(++):18, Eyes with dry-eye were K (-):3, K (+/-):8, K (+) 13, K (++):11, K ( ):5, and G (-):7, G (+/-):7, G (+):22, G (++):4. Clinical severity and the rose Bengal score corresponded to these categories. This technique can detect cytological changes in conjunctival cells in terms of shape and quality of staining. Eyes with dry-eye could be evaluated in relation to the numbers of keratinized cells and goblet cells.