Related Experiment Video
Updated: Jul 20, 2026

Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
Published on: July 6, 2016
Perylene-3,4:9,10-tetracarboxylic acid bisimide dye as an artificial DNA base surrogate
Clemens Wagner1, Hans-Achim Wagenknecht
1University of Regensburg, Institute for Organic Chemistry, Universitätsstr. 31, D-93053 Regensburg, Germany.
Abstract:
A phosphoramidite of the perylene bisimide dye was synthesized as a DNA building block that allows incorporation of this chromophore as an artificial nucleoside surrogate either at the 5'-terminus or at internal positions of duplex DNA. The internally incorporated perylene bisimide chromophore shows strong interactions with the DNA base stack; the 5'-terminally attached perylene bisimide is able to induce dimerization of two whole DNA duplexes.
More Related Videos
Related Concept Videos
DNA Base Pairing
DNA Base Pairing
Maxam-Gilbert Sequencing
Challenges of the Maxam-Gilbert Method
The...
Labeling DNA Probes
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
Base-pairing and DNA Repair
The DNA Helix

