Pseudomonas pohangensis sp. nov., isolated from seashore sand in Korea

A novel bacterium, designated strain H3-R18(T), was isolated from seashore sand collected from Homi cape, Pohang city, Korea. Cells were Gram-negative, aerobic, non-motile, cream-coloured, mesophilic and slightly halotolerant. 16S rRNA gene sequence analysis indicated that the organism was a member of the genus Pseudomonas, but the sequence showed < or =96.3 % sequence similarity to that of the type strains of all recognized Pseudomonas species. Highest sequence similarities were to Pseudomonas brenneri CFML 97-391(T) (96.3 %) and Pseudomonas migulae CIP 105470(T) (96.3 %). The major fatty acids were summed feature 3 and C(16 : 0), with lesser amounts of C(12 : 0), C(12 : 0) 3-OH, C(18 : 1)omega7c and C(14 : 0). The major isoprenoid quinone was Q-9. The DNA G+C content was 64.0 mol%. The phylogenetic, phenotypic and genetic properties of strain H3-R18(T) suggest that it represents a novel species, for which the name Pseudomonas pohangensis sp. nov. is proposed. The type strain is H3-R18(T) (=KACC 11517(T)=DSM 17875(T)).