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Intracellular localization of pp60c-src in human platelets.
J E Ferrell1, J A Noble, G S Martin
1Division of Biochemistry and Molecular Biology, University of California, Berkeley 94720.
Oncogene
|July 11, 1990
Summary
The proto-oncogene product pp60c-src is primarily located in human platelet membranes. This finding suggests its role in transmitting signals from cell surface proteins to intracellular targets.
Area of Science:
- Cell Biology
- Molecular Biology
- Oncology
Background:
- The proto-oncogene product pp60c-src is a key protein involved in cellular signaling pathways.
- Understanding the precise localization of pp60c-src is crucial for elucidating its function in cell regulation.
Purpose of the Study:
- To investigate the intracellular distribution of pp60c-src within human platelets.
- To determine the specific membrane compartments with which pp60c-src associates.
Main Methods:
- Immunofluorescence microscopy was employed to visualize pp60c-src.
- Immunogold electron microscopy on frozen thin sections provided high-resolution localization data.
Main Results:
- pp60c-src was predominantly found associated with the plasma membrane in human platelets.
- Significant localization of pp60c-src was also observed on the membranes of the surface-connected canalicular system.
Conclusions:
- The plasma membrane and surface-connected canalicular system are key locations for pp60c-src in platelets.
- This localization supports a role for pp60c-src in relaying extracellular signals to intracellular components.