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Published on: September 29, 2019
Analysis of protein S-100B in serum: a methodological study
Kay Müller1, Astrid Elverland, Bertil Romner
1Department of Neurosurgery, University Hospital of North Norway, Tromsø, Norway. kay.muller@unn.no
Clinical Chemistry and Laboratory Medicine
|September 9, 2006
Summary
Protein S-100B is not stable in stored serum, showing increased levels over time. The Elecsys S100 and Liaison Sangtec 100 tests are not interchangeable for measuring this biomarker.
Area of Science:
- Biochemistry
- Clinical Chemistry
- Neuroscience
Background:
- Protein S-100B is a key biochemical marker for central nervous system dysfunction and damage.
- Established methods exist for its detection, but long-term stability and new assay comparisons are needed.
Purpose of the Study:
- To evaluate the long-term stability of protein S-100B in frozen serum.
- To establish reference values for the Elecsys S100 assay.
- To compare the Elecsys S100 test with the Liaison Sangtec 100 assay.
Main Methods:
- Analysis of blood samples from 118 blood donors and 196 patients.
- Repeated analysis of frozen serum samples (1997 and 2003) to assess long-term stability.
- Method comparison using Bland-Altman plots for Liaison Sangtec 100 and Elecsys S100 tests.
Main Results:
- Serum S-100B concentrations significantly increased during long-term storage (mean difference 0.15 microg/L).
- Reference values for healthy blood donors using Elecsys S100: 0.02-0.08 microg/L (mean 0.05 microg/L, 95th percentile 0.07 microg/L).
- The Liaison Sangtec 100 test yielded higher concentrations than Elecsys S100, with method differences increasing at higher concentrations.
Conclusions:
- Protein S-100B is unstable during long-term serum storage.
- The Elecsys S100 and Liaison Sangtec 100 analytical methods are not interchangeable.
