[A method of determining interleukin-1 in a culture of lymphocytes and human blood monocytes activated by a pulsed method]
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Updated: Aug 8, 2026
Accurate and Simple Measurement of the Pro-inflammatory Cytokine IL-1β using a Whole Blood Stimulation Assay
Published on: March 2, 2011
Abstract:
Human peripheral blood monocyte monolayer was treated with lipopolysaccharide by the pulsed method. As a result of such activation the cells could produce interleukin-1, that enhanced the proliferation of lymphocytes added to the activated monocyte culture together with suboptimal dose of phytohemagglutinin.
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