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Updated: Jul 20, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials
Published on: July 3, 2011
Simultaneous saccharification and fermentation of cassava bagasse for L-(+)-lactic Acid production using Lactobacilli
Rojan P John1, K Madhavan Nampoothiri, Ashok Pandey
1Biotechnology Division, Regional Research Laboratory (CSIR) Trivandrum-695 019 Kerala, India.
Abstract:
Saccharification and fermentation of cassava (Manihot esculenta) bagasse was carried out in a single step for the production of L-(+)-lactic acid by Lactobacillus casei and Lactobacillus delbrueckii. Using 15.5% w/v of cassava bagasse as the raw material, a maximum starch to lactic acid conversion of 96% was obtained with L. casei with a productivity rate of 1.40 mg/mLxh and maximum yield of 83.8 mg/mL. It was 94% with L. delbrueckii with a productivity rate of 1.36 mg/mLxh and maximum yield of 81.9 mg/mL. Supplementation of bagasse with 0.01% w/v MnCl2 showed positive influence on the lactic acid production by L. casei.
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