Related Experiment Video
Updated: Jul 20, 2026

RNA Fluorescence In Situ Hybridization for Long Non-Coding RNA Localization in Human Osteosarcoma Cells
Published on: June 16, 2023
Expression of transcription factor Yin Yang 1 in human osteosarcomas
Filomena de Nigris1, Chiara Botti, Anna de Chiara
1Department of General Pathology, Division of Clinical Pathology, 1st School of Medicine, II University of Naples, Italy.
Abstract:
The transcription factor Yin Yang 1 (YY1) is known to be present in some human cancer cell lines and its expression correlates with immune-mediated apoptosis. By using Western blot analysis, we have shown that the YY1 protein is strongly expressed in human osteosarcoma cells and localised mainly in the nucleus. Moreover, by using immunohistochemistry and RT-PCR techniques, we have analysed the expression of YY1 protein in biopsies from human osteosarcomas. The YY1 protein was not detectable by immunohistochemistry in osteoid tissue. However, its expression was restricted to osteosarcoma tissues. These data were confirmed by densitometric analysis of RT-PCR for YY1 expression. Thus, YY1 gene activation appears to be an early event in the process of osteoblastic transformation and its detection may represent, together with the analysis of other established markers, a useful diagnostic tool in human osteosarcomas.
Related Concept Videos
Master Transcription Regulators
General Transcription Factors
Abnormal Proliferation
TGF - β Signaling Pathway
Methods of Nuclear Reprogramming
Transcription Factors

