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Functional correlation between kallikrein and factor XII activated in human plasma
K Briseid1, N O Hoem, S Johannesen
1Department of Pharmacology, University of Oslo, Blindern, Norway.
Thrombosis Research
|March 15, 1990
Summary
This study suggests plasma kallikrein and FXIIa form an association. Experiments show functional kallikrein and reduced FXIIa levels when these proteins interact in plasma.
Area of Science:
- Biochemistry
- Hematology
- Proteomics
Background:
- Plasma kallikrein and FXIIa are key enzymes in the contact activation system.
- Understanding their interactions is crucial for hemostasis and thrombosis research.
Purpose of the Study:
- To investigate the potential association between plasma kallikrein and FXIIa.
- To characterize the functional state of these enzymes during interaction.
Main Methods:
- Chromogenic substrate assays (S-2222) to measure enzyme activity.
- Inhibition studies using soybean trypsin inhibitor (STI) and corn inhibitor (C.I.).
- Gel filtration and PAGE immunoblotting to analyze protein complexes and molecular weights.
Main Results:
- Short incubation assays showed kallikrein inhibition by STI.
- Longer assays revealed functional kallikrein and reduced FXIIa, suggesting an association.
- Gel filtration indicated kallikrein co-eluted with high molecular weight kininogen, with FXIIa detected separately.
Conclusions:
- Evidence supports an association between plasma kallikrein and FXIIa.
- This interaction influences the functional state and activity of both enzymes.
- Further research is needed to elucidate the precise nature of this complex.