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Related Experiment Videos

Hyperspectral imaging system using acousto-optic tunable filter for flow cytometry applications.

Paul M Kasili1, Tuan Vo-Dinh

  • 1Advanced Biomedical Science and Technology Group, Oak Ridge National Laboratory, Oak Ridge, TN 37831, USA.

Cytometry. Part a : the Journal of the International Society for Analytical Cytology
|September 14, 2006
PubMed
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Hyperspectral imaging (HSI) can be integrated with flow cytometry, a cell analysis technique. This combination provides enhanced spatial and spectral data, improving multiparameter measurements for advanced biological research.

Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry
  • Spectroscopy

Background:

  • Flow cytometry offers flexible instrument configuration, enabling integration with auxiliary technologies.
  • This flexibility allows for the measurement of parameters beyond standard flow cytometry capabilities.
  • Hyperspectral imaging (HSI) presents a potential add-on for flow cytometry.

Purpose of the Study:

  • To explore the principle and application of integrating HSI with flow cytometry.
  • To develop and demonstrate an HSI system for flow cytometry that provides spectral and spatial information.
  • To assess the utility of HSI as an enhancement for multiparameter flow cytometry.

Main Methods:

  • Developed an HSI system for flow cytometry applications.

Related Experiment Videos

  • Acquired spatial information by recording 2D images at specific wavelengths.
  • Generated spectral information by obtaining full spectra from single sample sites within a defined wavelength range.

    • Main Results:

    • The integrated HSI system successfully provided both spectral and spatial data.
    • Demonstrated the acquisition of detailed spectral information from sample sites.
    • Showcased the ability to capture spatial imaging data within the flow cytometry workflow.

    Conclusions:

    • HSI is a promising add-on feature for flow cytometry.
    • The integration provides complementary spatial and spectral information to existing multiparameter data.
    • This enhancement expands the analytical capabilities of flow cytometry for complex biological samples.