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Updated: Aug 12, 2026

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections
09:11

Immunohistochemical Analysis in the Rat Central Nervous System and Peripheral Lymph Node Tissue Sections

Published on: November 14, 2016

Immunohistochemistry

S M Hsu

    Methods in Enzymology
    |January 1, 1990
    PubMed

    The use of the ABC technique, in which an avidin-biotin interaction is used for immunohistochemical studies, is a simple and straightforward procedure. All required reagents are commercially available. The main advantages of this technique are its sensitivity, specificity, and flexibility. It is crucial for the success of this staining procedure that the antibodies, the type of tissues, and the fixatives that are used be selected properly. The method can easily be adapted for electron microscopy, flow cytometry, in situ hybridization, Western blotting, and ELISA.

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