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Isolation and Characterization Of Chimeric Human Fc-expressing Proteins Using Protein A Membrane Adsorbers And A Streamlined Workflow
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A high-yield one-step purification method using copper-chelating chromatography for recombinant proteins fused with

Ka-Ming Lee1, Kit-Wan Ma, Pang-Chui Shaw

  • 1Molecular Biotechnology Program, The Chinese University of Hong Kong, Shatin, Hong Kong, China.

Analytical Biochemistry
|September 15, 2006
PubMed
Abstract

No abstract available in PubMed .

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Affinity Chromatography

Affinity chromatography is a powerful technique extensively utilized for separating and purifying specific biomolecules from complex mixtures. It capitalizes on the highly selective binding between an analyte and its counterpart, such as antibody-antigen interactions. The counterpart is immobilized on the stationary phase, forming an affinity column. The stationary phase typically consists of solid support, such as agarose or porous glass beads, immobilizing the affinity ligand. The mobile...
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The stability and compatibility of column material with samples are crucial for efficient purification in chromatographic techniques. Various operating parameters such as pH, temperature, or solvent affect the packing of the column material, thereby determining the purification efficiency. The choice of column material also plays an essential role in deciding the operating parameters and can be modified based on the proteins that need to be purified.
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