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Zymographic screening of plant peroxidase isoenzymes oxidizing 4-hydroxystilbenes
A A Calderón1, M A Pedreño, A Ros-Barceló
1Department of Plant Biology, University of Murcia, Spain.
Electrophoresis
|June 1, 1990
Abstract:
A zymographic assay is described for the detection of peroxidase isoenzymes oxidizing 4-hydroxystilbene following isoelectric focusing. The assay is based on coupling intermediate products of the oxidation of 4-hydroxystilbene with 4-aminoantipyrine, with resultant formation of dye complexes. Control experiments in the absence of 4-hydroxystilbene and hydrogen peroxide demonstrate the peroxidative nature of the 4-hydroxystilbene-dependent dye reaction.