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Related Experiment Video

Updated: Jul 20, 2026

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

Published on: September 21, 2010

The development of migrating muscle precursor cells.

Elena Vasyutina¹, Carmen Birchmeier

¹Max-Delbrück-Center for Molecular Medicine, Robert-Rössle-Str 10, 13125, Berlin, Germany.

Anatomy and Embryology

|September 16, 2006

Summary

Long-range migrating precursor cells from the dermomyotome form major hypaxial muscles. Proper specification, delamination, migration, and proliferation are essential for limb muscle development.

Area of Science:

Developmental biology
Molecular biology
Genetics

Background:

Hypaxial muscles are crucial for body movement and posture.
A specific subclass of hypaxial muscles originates from migrating precursor cells.

Purpose of the Study:

To investigate the developmental process of migrating precursor cells in hypaxial muscle formation.
To identify key factors and stages critical for the development of this muscle lineage.

Main Methods:

Utilized the homeobox gene Lbx1 as a marker for visualizing migrating precursor cells.
Studied the process in mouse models to understand cell specification, delamination, migration, and proliferation.

Main Results:

Migrating precursors are generated at specific axial levels (occipital, cervical, limb buds).

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Published on: April 30, 2014

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Adult and Embryonic Skeletal Muscle Microexplant Culture and Isolation of Skeletal Muscle Stem Cells

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Published on: September 21, 2010

Purification of Progenitors from Skeletal Muscle

Purification of Progenitors from Skeletal Muscle

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Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

Preparation of Primary Myogenic Precursor Cell/Myoblast Cultures from Basal Vertebrate Lineages

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These precursors give rise to limb muscles, the hypoglossal chord, and the diaphragm.

The homeobox gene Lbx1 is a reliable marker for these migrating cells.

Conclusions:

The development of migrating precursor-derived muscles relies on precise precursor specification and coordinated migration.
Cell proliferation at target sites is vital for achieving the correct muscle size.