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Related Experiment Videos

Mycobacterium cookii sp. nov.

J Kazda1, E Stackebrandt, J Smida

  • 1Division of Veterinary Medical Microbiology, Research Institute Borstel, Federal Republic of Germany.

International Journal of Systematic Bacteriology
|July 1, 1990
PubMed
Summary
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Systematic and applied microbiology·2012

New Zealand

Area of Science:

  • Microbiology
  • Bacteriology
  • Environmental Science

Background:

  • Scotochromogenic mycobacteria are a group of bacteria known for their slow growth and pigment production.
  • These organisms are often found in environmental sources, but their specific roles and diversity are not fully understood.

Purpose of the Study:

  • To characterize novel scotochromogenic mycobacterial strains isolated from New Zealand moors.
  • To determine the taxonomic and phylogenetic placement of these new isolates.
  • To assess the pathogenicity and immunological properties of the novel strains.

Main Methods:

  • Isolation and cultivation of mycobacterial strains from sphagnum vegetation and surface water.
  • Biochemical characterization including enzyme activities and tolerance tests.

Related Experiment Videos

  • Numerical taxonomy for strain similarity assessment.
  • Analysis of whole-mycolate patterns.
  • 16S ribosomal RNA (rRNA) gene sequencing for phylogenetic analysis.
  • Pathogenicity testing in animal models (mice, guinea pigs, rabbits).

    • Main Results:

    • Novel scotochromogenic mycobacterial strains were repeatedly isolated from New Zealand moors.
    • The isolates exhibited unique growth characteristics (growth at 22°C and 31°C, but not 37°C) and biochemical profiles.
    • Numerical taxonomy indicated high internal similarity (96.6% +/- 3.09%) among the strains.
    • Unique whole-mycolate patterns and 16S rRNA gene sequences confirmed their placement within the genus Mycobacterium, but in a unique phylogenetic position.
    • The strains were nonpathogenic in animal models but induced a hypersensitivity reaction to bovine tuberculin.

    Conclusions:

    • The novel strains represent a new species of nonpathogenic, slowly growing mycobacteria.
    • The proposed name for this new species is Mycobacterium cookii.
    • The type strain, NZ2 (ATCC 49103), has been deposited for future reference and study.