[Efficiency of assessment of retinol, alpha-tocopherol and beta-carotene by the liquid chromatography method]

Benign prostatic hyperplasia is very frequent disease of the primogenital system of elderly men. As the investigations show the antioxidant vitamins have various influence on the men's organism. They participate in the pathogenesis of many diseases. Taking into account all these circumstances, the prophylactic problem must be solved according to the foreseen provision of organism with the vitamins. It is very important to have the appropriate methods for determination of vitamins and their provitamins. Nowadays, to determine vitamin A, its form b-carotene and vitamin E, a High Pressure Liquid chromatography is used. We have developed an original, highly sensitive method of determining retinol, alpha-tocopherol and b-carotene, which is characterised by simplicity and high accuracy. Applying this method to the determination of vitamins concentration in 10 healthy donor's blood gave the following results: for the Vitamin A 567.99+/-57.49345 mkg/l , Vit. E-16.133+/-0.33941 mkg/l and b-carotene- 0.8621+/-0.810 mkg/l. This corresponds to the reference data. It is suggested that it is possible to use above-mentioned method for routine epidemiological investigations.