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[Effects of peritoneal dialysis solution on proliferation and apoptosis of rat peritoneal mesothelial cells]
Sheng-xiao Chen1, Hao Ren, Zheng-rong Liu
1Department of Nephrology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. csx@fimmu.com
Objective:
To investigate the effects of peritoneal dialysis solution (PDS) on proliferation and apoptosis of rat peritoneal mesothelial cells (RPMCs).
Methods:
The proliferation of RPMCs treated with PDS containing glucose of different concentrations for different times in vitro were examined by MTT colorimeric assay. The cell cycles and cell apoptosis rate were determined by flow cytometry.
Results:
After PDS treatment for 1 h, the cell proliferation inhibition rate, percentage of cells at G(0)/G(1) stage and early cell apoptosis rate increased, and such increment was more manifest with higher glucose concentration in PDS and longer treatment time of the cells. After treatment with PDS containing 4.25% glucose for 3 h, the proliferation inhibition rate of the RPMCs reached 44.12%, the percentage of cells at the G(0)/G(1) stage amounted to 71.95% and the early apoptosis rate reached 23.59%, which was several times higher than that of the negative control and 1.5% glucose/PDS groups, and also higher than that of 4.25% mannitol/PDS groups.
Conclusion:
PDS containing high-concentration glucose can induce significant apoptosis and inhibit the proliferation of RPMCs in vitro.
Insights
High-glucose peritoneal dialysis solution (PDS) significantly inhibits rat peritoneal mesothelial cell (RPMC) proliferation and induces apoptosis in vitro, with effects increasing with glucose concentration and treatment duration.
Area of Science:
- Cell Biology
- Renal Physiology
Context:
- Peritoneal dialysis is a common treatment for kidney failure.
- Peritoneal dialysis solutions (PDS) contain glucose or icodextrin as osmotic agents.
- Mesothelial cells lining the peritoneum are crucial for dialysis function.
Purpose:
- To investigate the impact of PDS, specifically varying glucose concentrations, on rat peritoneal mesothelial cells (RPMCs).
- To assess the effects of PDS on RPMC proliferation and apoptosis.
- To determine the relationship between glucose concentration, treatment duration, and cellular response.
Summary:
- RPMCs were exposed to PDS with different glucose concentrations and durations in vitro.
- Proliferation was measured using MTT assay, while cell cycle and apoptosis were analyzed by flow cytometry.
- High-glucose PDS (4.25%) significantly inhibited proliferation, increased G(0)/G(1) phase arrest, and elevated early apoptosis rates compared to controls and lower glucose concentrations.
Impact:
- High glucose concentrations in PDS can adversely affect peritoneal membrane health by inhibiting mesothelial cell proliferation and inducing apoptosis.
- These findings suggest potential mechanisms for peritoneal membrane dysfunction in patients undergoing long-term PD with high-glucose solutions.
- Further research may inform the development of PDS with improved biocompatibility to preserve peritoneal function.
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