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Related Experiment Videos

Hexose transport in sarcoma virus transformed cells.

M Hatanaka, J C Graff

    Bibliotheca Haematologica
    |January 1, 1975
    PubMed
    Summary

    Sarcoma virus transformation dramatically enhances hexose transport in cells, independent of metabolism. Sugars inhibiting this transport in vitro also reduced sarcoma incidence in vivo, suggesting transport manipulation for cancer control.

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    Area of Science:

    • Cell Biology
    • Virology
    • Biochemistry

    Background:

    • Cellular transformation by sarcoma viruses induces significant physiological changes.
    • Understanding alterations in nutrient transport is crucial for cancer research.

    Purpose of the Study:

    • To investigate the changes in hexose transport rates following sarcoma virus transformation.
    • To determine if altered hexose transport is linked to malignancy and can be targeted for therapeutic intervention.

    Main Methods:

    • Utilized non-metabolizable glucose analogues (2-deoxyglucose, 3-O-methylglucose, L-glucose) to study hexose transport.
    • Assessed hexose transport in both transformed and non-transformed avian and mammalian fibroblast cultures.
    • Investigated the effect of specific sugars on sarcoma virus transformation in vitro and in vivo.

    Main Results:

    • Sarcoma virus transformation led to a rapid and significant enhancement of hexose transport, distinct from changes in non-transformed cells.
    • Enhanced transport was independent of overall glucose metabolism and hexokinase activity.
    • Transformed cells exhibited altered transport characteristics, including increased 3-O-methylglucose uptake even with cytochalasin B and L-glucose transport, which was absent in control cells.
    • Certain sugars inhibited sarcoma virus transformation in vitro and reduced sarcoma incidence in vivo.

    Conclusions:

    • Sarcoma virus transformation profoundly alters cellular hexose transport systems.
    • The observed changes in hexose transport correlate with the transformed phenotype.
    • Targeting these altered transport systems presents a potential strategy for controlling certain malignancies.

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