Related Experiment Videos
[Mechanism of interaction of DNA with fluorescent dye Hoechst 33258]
Biofizika
|May 1, 1990
Abstract:
From the study of absorption and fluorescence spectra of the complex "Hoechst-33258"--DNA at different pH it is shown that AT--specific complex with DNA is formed by the neutral dye molecule, whereas the cationic state of the dye molecule forms the nonspecific complex. Possible formation of a specific complex in which the dye is bound to DNA in its major groove is discussed.