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Related Experiment Videos

Assembling microtubules disintegrate the postsynaptic density in vitro.

Li-Ping Lo1, Szu-Heng Liu, Yen-Chung Chang

  • 1Department of Life Science, Institute of Molecular Medicine, National Tsing Hua University, Hsinchu, Taiwan, Republic of China.

Cell Motility and the Cytoskeleton
|September 21, 2006
PubMed
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Interactions between assembling microtubules and postsynaptic densities (PSDs) alter PSD structure. This may lead to hole formation and fragmentation of PSDs in the mammalian central nervous system.

Area of Science:

  • Neuroscience
  • Cell Biology
  • Biochemistry

Background:

  • The postsynaptic density (PSD) is a critical protein complex in excitatory synapses of the mammalian central nervous system (CNS).
  • PSDs are involved in signal transduction and molecular organization within dendritic spines.
  • Microtubules have been observed transiently in the PSD-enriched cytoplasm of dendritic spines.

Purpose of the Study:

  • To investigate the structural effects of interactions between assembling microtubules and isolated postsynaptic densities (PSDs).
  • To understand how microtubules influence the integrity and organization of PSDs.

Main Methods:

  • Incubation of isolated porcine brain PSDs with polymerizing alpha,beta-tubulins.
  • Transmission electron microscopy (TEM) to visualize structural changes in PSDs.

Related Experiment Videos

  • Comparison of PSDs incubated with assembling microtubules versus those incubated with pre-treated tubulin (vincristine).

    • Main Results:

    • Incubation with assembling microtubules increased PSD turbidity, indicating structural alteration.
    • TEM revealed novel structures like holes and finger-like extensions in PSDs after microtubule incubation.
    • Smaller PSD fragments were observed, suggesting a breakdown of the PSD structure.
    • No significant structural changes were observed in PSDs incubated with pre-treated tubulin.

    Conclusions:

    • Assembling microtubules interact with PSDs, inducing structural changes.
    • These interactions can lead to the formation of holes within the PSD.
    • The rupture of these holes may cause PSD fragmentation, impacting synaptic structure and function.